panmeibing Sorry for the confusion. The following video demonstrates the steps to set up the sequence correctly:

What is important is that the name before the sequential number of the image is specified in the Image path, and then the frame number is entered so that the digits match exactly, including 0 when entering the start frame. Since your sequence image is 3 digits, like 001, if the digits do not match, the image cannot be referenced correctly.

panmeibing Please correct the Image path field. In the case of the one you showed in your video, it should be possible to successfully reference the image if only s01/ is entered.

panmeibing The Spineboy example I posted in my previous answer was recorded using Spine 4.1, so the sequence will work fine in 4.1 as long as the image path is correct and the appropriate frame number is specified. If you don't mind, you can send us the actual Spine project files via email, then we can check the correct image path and frame number combination and get back to you: contact@esotericsoftware.com
Please include the URL of this forum thread in the email so we know the context. Then we can take a look at what's wrong.

panmeibing To remove an editor version that you have already downloaded, delete the file for each version in the update folder in your user folder. The location of the user folder varies depending on the operating system you are using, so please see the following page for more information:
Spine故障排除 #用户文件